Formation of Thiolated Nucleosides in tRNA of Salmonella enterica Serovar Typhimurium
نویسنده
چکیده
..................................................................................................................................... 4 Summering ................................................................................................................................. 5 Publication list............................................................................................................................ 6 Introduction ................................................................................................................................ 7 Translation.............................................................................................................................. 7 Translational proofreading and fidelity............................................................................ 10 Translational errors and frameshifting ............................................................................. 11 Modified nucleosides ........................................................................................................... 13 Thiolated nucleosides in tRNA ............................................................................................ 16 sU .................................................................................................................................... 17 sC .................................................................................................................................... 17 mnmsU/cmnmsU ........................................................................................................ 18 msioA............................................................................................................................. 18 Iron sulfur metabolism in enterobacteria ............................................................................. 19 Iron sulfur clusters............................................................................................................ 19 The isc-operon.................................................................................................................. 21 Assembly pathway ........................................................................................................... 22 IscA .................................................................................................................................. 23 Suf .................................................................................................................................... 24 The working model .......................................................................................................... 24 Tools of the trade.................................................................................................................. 26 Choice of model organism ............................................................................................... 26 Aims ......................................................................................................................................... 28 Results ...................................................................................................................................... 29 Paper I .................................................................................................................................. 29 Paper II ................................................................................................................................. 31 Paper III................................................................................................................................ 35 Discussion ................................................................................................................................ 36 References ................................................................................................................................ 38 Acknowledgements .................................................................................................................. 47
منابع مشابه
Formation of thiolated nucleosides present in tRNA from Salmonella enterica serovar Typhimurium occurs in two principally distinct pathways.
tRNA from Salmonella enterica serovar Typhimurium contains five thiolated nucleosides, 2-thiocytidine (s(2)C), 4-thiouridine (s(4)U), 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U), 5-carboxymethylaminomethyl-2-thiouridine (cmnm(5)s(2)U), and N-6-(4-hydroxyisopentenyl)-2-methylthioadenosine (ms(2)io(6)A). The levels of all of them are significantly reduced in cells with a mutated iscS gene, wh...
متن کاملMultilocus Sequence Typing of the Clinical Isolates of Salmonella Enterica Serovar Typhimurium in Tehran Hospitals
Background: Salmonella enterica serovar Typhimurium is one of the most important serovars of Salmonella enterica and is associated with human salmonellosis worldwide. Many epidemiological studies have focused on the characteristics of Salmonella Typhimurium in many countries as well as in Asia. This study was conducted to investigate the genetic characteristics of Salmonella Typhimurium using m...
متن کاملThe cysteine desulfurase IscS is required for synthesis of all five thiolated nucleosides present in tRNA from Salmonella enterica serovar typhimurium.
Deficiency of a modified nucleoside in tRNA often mediates suppression of +1 frameshift mutations. In Salmonella enterica serovar Typhimurium strain TR970 (hisC3737), which requires histidine for growth, a potential +1 frameshifting site, CCC-CAA-UAA, exists within the frameshifting window created by insertion of a C in the hisC gene. This site may be suppressed by peptidyl-tRNAProcmo5UGG (cmo(...
متن کاملمقایسه پلیمورفیسم ژنومی و ارتباط ژنتیکی سویههای بالینی سالمونلا انتریکا سرووار تیفی موریوم در استان کرمان به روش ERIC- PCR و Box-PCR
Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method. Methods:...
متن کاملEffect of Electron Beam Irradiation on Survival of Escherichia coli O157:H7 and Salmonella enterica serovar Thyphimurium in Minced Camel Meat during Refrigerated Storage
Background: Electron beam irradiation is one of the effective ways to control food-borne pathogens. We evaluated the effect of electron beam irradiation on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Thyphimurium in minced camel meat during refrigerated storage. Methods: The meat samples were inoculated with E. coli O157:H7 and S. enterica serovar Thyphimurium and then...
متن کامل